摘要
青霉素结合纳米颗粒能否恢复Ã Â -内酰胺类抗生素对耐甲氧西林金黄色葡萄球菌的活性?
作者(年代):s。m。abdelhamid, h。s。ramadan, n。a。mokhless, n。a。abo - khedr常用抗生素如青霉素和其他â-lactam抗MRSA药物的失效,导致迫切需要改进药物设计、发现和交付。纳米技术在给药系统中的应用被广泛期待在可预见的未来改变制药和生物技术行业的格局,其中纳米颗粒代表了非常有前途的壳聚糖方法来实现这一目标。这项工作的目的是评估青霉素结合壳聚糖纳米颗粒是否对MRSA表现出抗菌活性,并确定青霉素结合纳米颗粒的生物活性。采用离子凝胶法制备壳聚糖纳米颗粒,并采用掺入法在纳米颗粒加工过程中负载青霉素。利用透射电镜、粒度分析仪和药物包封率对制备的纳米颗粒进行了表征。金黄色葡萄球菌分离株采用唑西林和头孢西丁圆盘扩散法,PCR检测mecA基因或鉴定mrsa和mssa分离株。筛选出50株mrsa和30株MSSA进行进一步检测。采用微量肉汤稀释法测定青霉素及青霉素结合壳聚糖纳米颗粒MIC含量。所有MRSA菌株均对两种方法均有耐药性。对于MSSA分离株,使用青霉素结合的壳聚糖纳米颗粒,在稀释e - 512µg/ml(0.0%)时,没有一株表现出耐药性,10株(33%)显示为e - 16µg/ml, 7株显示为16µg/ml。 It was observed that 3 isolates (10%) of MSSA turned sensitive to penicillin when performing the MIC broth microdilution test, using penicillin-bound chitosan nanoparticles. Though 27 MSSA isolate remained resistant; yet the MIC of penicillinbound chitosan nanoparticles was significantly reduced. In conclusion, penicillin-bound chitosan nanoparticles was effective only with MSSA producing penicillinase in reducing MIC of penicillin or even making MSSA sensitive to penicillin. But with MRSA, penicillin-bound chitosan nanoparticles gave no effect.
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