摘要
宽Entrococcus hirae丝氨酸蛋白酶基因的克隆、表达及特性研究
作者(年代):Chandran Masi, P.Vivek, J.Kotteshwari, C.Mithun, A.Uma Maheswaran, N.Parthasarathi平肠球菌是一种革兰氏阳性菌。平肠球菌具有产生蛋白酶的能力。在本实验中,我们尝试从hiraee肠球菌中获取蛋白酶产生基因,并将其转移到非蛋白酶产生生物中。这意味着通过本实验确定了哪个基因负责产生蛋白酶。引物设计工具用于设计DNA扩增的特异性引物。引物用于扩增。采用DNA分离法从平肠球菌中分离出DNA。用琼脂糖凝胶电泳法对分离的DNA进行交叉检查。将分离出的DNA进一步导入pcr机进行扩增。PCRMasterMix和引物用于扩增。After theDNAamplification, the cloning vectors are used for Cloning. The Ta plasmid vector (pBZ57RT) are used for DNA cloning, the cloned DNA with the vector is transformed into the Non protease producing organism, such as E.coli and checked for its activity.
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