文摘
比较分析和表征各种症状相关的人类胶原蛋白使用计算工具和在线服务器
作者(年代):K。Sivakumar, S。巴拉吉,恒河Radhakrishnan胶原蛋白是人体中最丰富的蛋白质。胶原蛋白形成结缔组织的重要组成部分,它可以被描述为支持组织器官的身体。胶原蛋白缺陷导致各种症状。在这里,我们报告的分析和表征研究各种综合症[Alport综合征(如),恰当牵拉(EDS)、肺出血肾炎综合症(GPS)和Stickler综合征(SS)] 13有关人类胶原蛋白从瑞士- Prot检索数据库对这些蛋白质提供更详细的描述。一级结构分析的结果表明,EDS和学生相关的胶原蛋白富含甘氨酸(23%到28%)和脯氨酸(15%到23%)残留。GPS相关的胶原蛋白富含Glu(9%)和Ser(9%)残留。胶原蛋白的计算π值P02452、P02461 P20908, P05997《经济学(季刊)》相关的蛋白质胶原Q9Y5P4 (GPS相关蛋白),胶原蛋白P12107, P13942 (SS相关的胶原蛋白)显示,这些都是酸性的性格(π< 7)。所有alport综合征相关的计算π人类胶原蛋白(P29400、P53420 Q01955和Q14031),蛋白质P08123 P02458 (EDS相关的胶原蛋白)和蛋白质(SS相关的胶原蛋白)推断,这些都是基本的性格(π> 7)。基本和酸性氨基酸的数量在每个胶原蛋白质关联与相应的计算π。低脂肪指数(37-59)的大部分症状相关的蛋白质推断胶原蛋白质在高温下可能会变得不稳定。 The biocomputed half-life of AS, GPS and SS associated collagen is 30hrs and greater than 20hrs for EDS associated collagens. Expasy’s ProtParam classifies the GPS associated collagen as unstable on the basis of instability index (II>40) and all the other collagen proteins as stable (II<40). Secondary structure analysis shows that all the collagens are found to be predominant coil structure content. The SSCP server classifies the GPS associated collagen (Q9Y5P4) as mixed secondary structure class and all the other collagens as irregular secondary structure class proteins. The irregular structure of collagen proteins is due to the rich content of more flexible glycine and hydrophobic proline amino acids. The server SOSUI classifies all the four AS associated collagen proteins and the protein P02458 (SS associated collagen protein) as membrane protein and all the other proteins as soluble proteins. The identified transmembrane regions were visualized and analyzed using helical wheel plot generated by EMBOSS pepwheel tool and it is found that the helical wheel consists of more hydrophobic residues. This is the first report on characterization and comparative studies of AS, EDS, GPS and SS associated human collagen proteins.
