文摘
快速液相色谱方法的开发和验证的同时估计nimesulide和盐酸tizanidine医药片剂剂型
作者(年代):Nitin D。Rawool, A.Venkatchalam本研究的目的是开发一个简单、精确和准确的反相高效液相色谱法和后续验证相同的按照我的指导方针。乐动KENO快乐彩本研究涉及的评估两种不同的药物成分的RP HPLC nimesulide (NMS)和盐酸tizanidine (TZN)出现在一个平板配方。NMS的色谱分离和TZN完成使用磷酸盐缓冲剂和甲醇作为流动相,在50的比例:50。C18柱分离完成,据估计在250 e max nmwith 1毫升/分钟的流量。保留时间为3.7和9.3分钟分别TZN和NMS。干扰的特异性检查任何峰主峰的兴趣。重复性检查了系统精度相对标准偏差小于1%在所有情况下,沿着withmethod TZN和NMS的再现性。线性范围从10.02至30.07毫克/毫升TZN和500.60 - 1501.80毫克/毫升NMS分别。回归方程的相关系数(r)在所有情况下都大于0.999。系统适用性精度也检查,以确保精确的分析方法。 The precision of the method was demonstrated using intra- and inter-day assay R.S.D. values which were less than 1%. The method was found to be accurate and precise for estimation of the two drugs simultaneously. According to the validation results, the proposed method was found to be specific, accurate, precise and rapid. Hence the same can be applied to the quantitative analysis of tablets containing NMSÂTZN binary mixtures.
