文摘

促黄体激素受体基因的遗传多态性与生育的埃及水牛

作者(年代):ASA索萨,卡莉玛Gh M艾哈迈迪,嗯Kandiel, HAA Eldebaky, MF Nawito和MEA阿布E l-Roos

本研究的目的是检测的遗传多态性促黄体激素受体(LHR)基因及其联系生育能力在埃及使用PCR-RFLP水牛和测序分析。总数301由100肥沃和201 non-fertile水牛。妇科检查(由超声辅助)进行了两次连续两个星期。从女性和提取的基因组DNA聚合酶链反应产品评估。不活跃的卵巢和子宫内膜炎的百分比(研究生,我)是最常见的生殖问题。在卵泡期平均子宫角最大直径是紧随其后的是黄体期和卵巢静止(2.67±0.11,2.28±0.09,2.18±0.11厘米,分别)。的聚合酶链反应产品的底漆特定的LHR基因给了特定的乐队在大小303个基点。的amplified fragments remained undigested by HhaI restriction enzymes and all tested buffalo were genotyped as TT. DNA sequence of 259 bp out of the amplified 303 bp was determined. The sequence alignment of 259 bp of Egyptian buffalo LHR with published sequence (accession number: XM_006078413.1 and JQ885687.1, Bubalus bubalis) showed that the 259 bp segment possess identities at 100%. In conclusion, monomorphic pattern for LHR gene is considered a unique feature that may be related to the characteristic物种在布法罗。因此,多态性和相互作用生育能力特征应该进一步研究的主题更大数量的动物。