摘要

两种水飞蓟素异构体对过敏性哮喘Balb/c小鼠模型的免疫调节作用

作者(年代):Seyyed Shamsadhin Athari

简介和目的:过敏性哮喘是一种以咳嗽、呼吸困难、喘息和气道阻塞为主要表现的复杂的呼吸系统慢性疾病。全世界各年龄段有3亿多人患有哮喘。免疫和炎症过程是哮喘的主要原因。辅助T 2淋巴细胞产生的细胞因子在哮喘的发生和发展中起主要作用。水飞蓟素是水飞蓟属植物的主要成分，是一种具有抗氧化作用的治疗药物。本文旨在比较两种水飞蓟素异构体异水飞蓟宾A和水飞蓟素在治疗过敏性哮喘中的抗炎和抗过敏作用。材料与方法:分离纯化异水飞蓟宾A和水飞蓟菊素后，用卵清蛋白注射液制备过敏性哮喘Balb/c小鼠模型。70只小鼠分为5组(正常组1只，哮喘组4只)(每组14只)。四组哮喘小鼠中的三组分别用异水飞蓟宾A、水飞蓟菌素或布地奈德治疗。第四组为阳性对照，非哮喘组为阴性对照。 Airway hyperresponsiveness (AHR) and levels of IL-4, IL-5 and IL-13 in the BAL fluid were determined. Gene expressions of IL-4, IL-5, IL-13 and MUC5ac, as well as IgE serum level were also measured. Cellular composition of BAL fluid and lungs histopathology were finally investigated. Results: Isosilybin A and silydianin reduced eosinophilic infiltration of lungs, IL-4 and IL-5 levelsin BAL fluid, IL-4 and IL-5 gene expressions, as well as AHR in Balb/c mouse model of asthma. However, no significant changes were observed in IL-13 level and mucus hyper-secretion. Conclusion: According to our study, isosilybin A and silydianin can control main symptoms of asthma by modulating immune responses.