文摘

响应面优化enzyme-assisted总黄酮类化合物的提取fromwisteria和研究羟基自由基清除效果

作者(年代):LiHui-duan

响应面方法(RSM)和Box-Benhnken设计(bdd)是用来优化从紫藤总黄酮类化合物的提取条件。羟基自由基清除和不活动清除和抑制效应的紫藤总黄酮类化合物也被研究。总黄酮类化合物提取从紫藤的脖子,叶,花和水果夹用enzyme-assisted乙醇回流。根据单因素实验结果,酶浓度0.15 ~ 0.25 mg·mL-1酶pH值5 ~ 7日酶温度45 ~ 65 !和酶时间1.5 ~ 2.5 h被选为bdd的独立变量和范围。RSM首选的提取条件进行优化。羟基自由基清除和抑制效应的紫藤提取物也被测量。RSM和bdd的最佳提取条件优化酶浓度为0.23 mg·mL-1;酶酶的pH值6日温度64 !和时间为2.0 h反应萃取率为0.94%紫藤的脖子,紫藤叶为4.60%,3.32%,紫藤鲜花为5.16%,紫藤水果夹。 The experimental extraction ratio matched well with the calculated values by solving the multiple regression equation. It confirms that the fitted quadratic model has a predictive effect on target extracts. The scavenging effect on hydroxyl radicals displayed a significant dose-effect relationship for Wisteria flavonoids, but it showed a weaker scavenging effect compare to BHT with the same concentration.