摘要

降糖防龙方对糖尿病耳聋动物模型一氧化氮合酶mrna表达的影响

作者(年代):郭凤学，马学玉，侯金杰，李斌，李恩，李瑞玉

目的:探讨降糖防龙方对糖尿病耳聋动物一氧化氮mRNA表达的影响。方法:采用链脲佐菌素(STZ)诱导的即刻糖尿病聋Wistars造模方法，从60只Wistars中随机抽取49只分为3组。A组有氨基胍组13例，B组有降糖方龙组13例，C组有病理组13例。A组wistar用稀释溶液重新配制成3%的氨基胍浓度喂养。B组饲喂降糖防龙混悬液。剩下的11名wistars是对照组。对照组11只wistar和C组13只wistar可以免费饮水。在20到25摄氏度的室温下，所有四组都吃了12周的正常食物。第6周和第12周测血糖、尿糖和体重。12周后，采用组织学a级反转录PCR技术，采用双能x线吸收法检测股骨、胫骨骨密度，观察骨组织及xun OsmRNA表达变化。结果:C组骨密度明显降低。 Degenerative changes occur in the osseous tissue. The expression of bone iNOsmRNA is increasing. Density in group B was obviously higher, significantly improving the bone histomorphology. the expression of bone iNOsmRNAwas decreased, which is similarly to Group A. Conclusion: The abnormal expression of iNOsmRNA in diabetic deafness will inhibit the expression of bone iNOsmRNA and may affect the kidney to advocate bone osteocalcin to insulin regulation. Therefore, speculating that hypoglycemic prevent deafness through kidney raising the function of “ kidney advocate bone “ can have effect on diabetes prevention and control of deafness. This speculation also provide an objective foundation for the scientific hypothesis of diabetes-kidneybone- ear integration theory.